Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating

Figure 8

ELISA distinguishes specific binding to HER2 protein. All nanomaterial, except the As-M-antibody, has an absorbance value < 0.20. Anti-HER2 scFv conjugated with As-M can detect HER2 protein, with a 1.95-fold difference compared with Dx-M-antibody. Dx-M (nonconjugate scFv) was used as the negative control. The results were obtained from triplicate determinations and are expressed as mean ± SD.