Denoising and Principal Component Analysis of Amplified Raman Spectra from Red Blood Cells with Added Silver Nanoparticles

Table 1

Principal component analysis results from RBC Raman spectra with and without AgNP.

Mean	Standard deviation	Eigenvalues	PC1 coefficient	CP2 coefficient	Variability (%)	Cumulative	PC 1	PC 2
RBC Raman spectra without AgNP
435.95	313.05875	79.03374	0.11001	−0.04796	83.19%	83.19%	−7.50495	−3.14694
481.68	358.32162	14.88045	0.10944	0.03865	15.66%	98.86%	−7.56427	−3.15832
417.55	331.27881	0.25699	0.11141	−0.00945	0.27%	99.13%	−7.35318	−3.22056
473.72	332.63329	0.10526	0.11058	0.02211	0.11%	99.24%	−7.00289	−3.28952
490.56	341.39515	0.07569	0.11131	0.01602	0.08%	99.32%	−6.71021	−3.31462
500.20	339.27534	0.0405	0.1097	0.04543	0.04%	99.36%	−6.58359	−3.31285
819.22	605.57175	0.02471	0.08893	0.15731	0.03%	99.39%	−6.62827	−3.30024
421.03	297.20846	0.02142	0.10817	−0.06154	0.02%	99.41%	−6.82522	−3.26039
371.24	294.55975	0.01972	0.10645	−0.08063	0.02%	99.43%	−7.16513	−3.17401
356.21	301.66804	0.01873	0.10604	−0.08307	0.02%	99.45%	−7.60888	−3.05894
RBC Raman spectra with AgNP
838.76	466.44873	77.80994	0.10796	0.0767	86.46%	86.46%	−9.0323	1.7079
589.22	390.11247	11.23373	0.09223	0.1709	12.48%	98.94%	−9.0674	1.74138
1237.61	1002.19047	0.29913	0.10315	−0.102	0.33%	99.27%	−9.0429	1.78999
727.51	460.3899	0.1151	0.1116	0.0472	0.13%	99.40%	−8.9426	1.85733
1116.57	756.02231	0.08343	0.10916	−0.0762	0.09%	99.49%	−8.8108	1.92021
801.73	519.32077	0.0572	0.11248	0.0167	0.06%	99.55%	−8.7346	1.93386
1486.09	1157.09662	0.04709	0.10314	−0.1222	0.05%	99.61%	−8.7759	1.87638
1171.28	850.75433	0.02201	0.10681	−0.0959	0.02%	99.63%	−8.9351	1.77626
740.12	474.83072	0.01593	0.11229	0.0261	0.02%	99.65%	−9.1801	1.67912
700.17	435.21653	0.01309	0.11164	0.0424	0.01%	99.66%	−9.4829	1.59353