Nanocrystalline Hydroxyapatite-Based Scaffold Adsorbs and Gives Sustained Release of Osteoinductive Growth Factor and Facilitates Bone Regeneration in Mice Ectopic Model

Figure 4

Representative images of histological tissue sections: (a) NHA, (b) L-BMP2-NHA, (c) M-BMP2-NHA, and (d) H-BMP2-NHA implants. (e) Higher magnification of image in (d). (f) Quantitative analysis of the BV/TV ratio. Scale bars in (a), (b), (c), and (d): 100 μm. Scale bar in (e): 50 μm. The sections were stained with Masson’s trichrome staining. The red arrows indicate the blue collagen fibers in the newly formed bone. The white arrows indicate the lacunae. The black arrows indicate the residual material. Data are presented as the mean ± SD, . Significant effect of the treatment compared to control, , and compared to L-BMP, . BV/TV: bone volume over total volume ratio.

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