Artificial plasma; 2.5-3.5 kg, sex-unlimited, 3-4-month-old, healthy, clean New Zealand white rabbits.
The corrosion rate of coated JDBM exposed to artificial plasma was reduced from to . Excellent tissue compatibility of adequately reendothelialized stent with no sign of thrombogenesis and restenosis in the stent-supported vessel.
Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (P/S), and 100 U/mL lipase solution; whole ovine blood for blood contacting test.
The PCUU-coated Mg stents exhibited improved corrosion resistance compared with the uncoated and PLGA-coated stents; the PCUU-coated Mg stents exhibited reduced thrombotic deposition versus the uncoated and PLGA-coated stents; the release of paclitaxel from the PCUU coating effectively inhibited rSMC proliferation.
The first and third layers were low molecular weight dextran loaded with sirolimus, and the second layer was PGA.
DC polarization and immersion tests; culturing cells for indirect cell viability and cell proliferation tests; hemolysis assay for blood compatibility.
Coating increased the corrosion resistance, cell viability, and proliferation rate and was nonhemolytic; the released sirolimus had no obvious effect on cell viability but could inhibit cell proliferation.
Iliac artery of New Zealand white rabbits with weight of 2.5-3.0 kg.
The corrosion rate was fast in PBS, deployment was safe, and endothelial coverage occurred after 42 days; the corrosion rate needs to be slower or the mechanical properties require improvement to scaffold the arteries for 183 days.
Coated Mg stents exhibited a dramatic corrosion rate improvement in electrochemical and immersion degradation tests for 14 days; coating created favorable environment for ECs to have competitive advantage over vascular smooth muscle cells.
Long-term immersion and electrochemical tests by standard Hank’s solution ( at 37°C); HUVEC and HASMC for in vitro cytocompatibility.
Arg-Leu-PEUU-coated Mg-Zn-Y-Nd alloys improved corrosion resistance, reduced hemolysis rate and platelet adhesion, and exhibited better cytocompatibility with better cellular morphology, proliferation, and biofunctionality in in vitro experiment.
Electrochemical measurements and long-term immersion tests; VSMC and HUVEC for cell adhesion and cell viability tests.
The anticorrosion capacity was greatly enhanced by coating; pre-treated PLGA coating as modified PLGA coating was effective in improving the corrosion behavior and biocompatibility.
