Research Article | Open Access
Green Synthesis of Nanofertilizers and Their Application as a Foliar for Cucurbita pepo L
The implementation of nanofertilizers in agriculture is the purpose in specific to decrease mineral losses in fertilizing and raises the yield during mineral management as well as supporting agriculture development. Hence, this experiment was conducted in Shebin El-Kom, El-Monifia governorate, Egypt, during two seasons 2017 and 2018 to study the effect of micronutrient oxide nanoparticles of zinc, iron, and manganese, as well as combination between these oxides as a foliar application on the growth, yield, and quality of squash plants. The obtained results showed that the spraying of manganese oxide nanoparticles on the plants led to the best vegetative growth characteristics, also, the characteristics of the fruits, yield, and the content of photosynthetic pigments. On the contrary, the content of organic matter, protein, lipids, and energy gave the highest value in squash fruits that have been sprayed with iron oxide nanoparticles.
Nanotechnology is an interdisciplinary promising research field, opening a vast number of opportunities in fields like medicine, pharmaceuticals, electronics, and agriculture. The term nanomaterials are generally used to describe the materials having a size between 1 and 100 nm. The small size and enormous surface area of such characteristics give unique properties for nanomaterials like optical, physical, and biological [1–3].
Recently, a wide range of nanotechnology applications has been intensively studied in the agriculture research sector developing practices at both academic and industrial levels [4–6]. In fact, nanotechnology has the potential to improve the entire current agricultural and food industry, by developing new tools for plant disease treatments , pathogen detection , and improving the ability of plants to absorb nutrients [9, 10]. Furthermore, nanotechnology started to attract more intention in the agriculture field especially to produce new nanofertilizers for increasing the efficacy and bioavailability of such new fertilizers as well as decreasing the loss of these materials to the surrounding environment . Many researchers illustrated that the agromorphological criteria, photosynthesis, and the yield of wheat plant  and common bean  were improved by the application of ZnO-NPs as a foliar fertilizer. Furthermore, Khodakovskaya et al.  found that carbon nanoparticles improve the plant criteria and the yield of the tomato.
Du et al.  represented that the ZnO-NPs were more significantly effective on the germination and growth of wheat rather than ZnSO4. In addition, they showed that ZnSO4 was more toxic than ZnO-NPs at higher dosages. In addition, Shaban et al.  showed that the common bean that is obtained from the plant treated by ZnO-NPs has no effect on the lipid parameters as well as the function of the kidney and liver of the rats that feed on this common bean.
Iron, manganese, and zinc are three essential elements for the growth of many plants including squash [16–19]. A microwave-assisted hydrothermal synthesis technique has been chosen for the preparation of these elements in the form of their respective oxides as nanofertilizers in this study. It is a widely used technique in many areas of chemistry especially in metal oxide nanoparticle synthesis [20, 21]. This method is a facile, fast, secure, controllable, and energy-saving process [22, 23]. It also provides an effective way to control particle size distribution and macroscopic morphology during the synthesis process .
This study is aimed at investigating the efficacy of such nanofertilizers towards the growth and yield of squash. The fertilization process was accomplished using each nanooxide alone; besides, their combinations contained two oxides together: zinc-iron, zinc-manganese, and manganese-iron, as well as the three nanooxides mixed together.
2. Materials and Methods
2.1. Preparation of Nanofertilizers
Ferric, manganese, and zinc nitrate analytical grade salts were used as precursor for the preparation of the nanooxide fertilizers, using a green microwave-assisted hydrothermal method. The desire amounts of Fe(NO3)3·9H2O, Mn(NO3)2·4H2O, or Zn(NO3)2·6H2O were dissolved in 50 mL water to obtain 0.2 M salt concentration. The pH of this solution was adjusted to 11 using 2 M NaOH solution and then transferred to 100 mL Teflon autoclave vessel. The vessel was then transferred to a 750 w advanced microwave synthesis lab station (Milestone MicroSYNTH). The microwave was adjusted to reach the desired temperature in 3 min, and then the temperature was held constant for 10 more minutes to ensure the production of the desired nanooxides. The holding temperatures were 100, 160, and 180°C for Mn3O4, ZnO, and Fe2O3, respectively. The obtained oxides were then washed 3 times with water to get rid of all the unreacted salts, dried at 100°C overnight, grinded, and then stored in a desiccator under anhydrous calcium chloride for further characterizations and studies.
2.2. Characterization of Nanofertilizers
A PHILIPS® X’Pert diffractometer, with the Bragg-Brentano geometry and copper tube, was used to collect the XRD patterns for the prepared nanooxide samples. The operating voltage was kept at 40 kV and the current at 30 mA. The divergence-slit , the receiving , the step scan , and the scan step . The Kβ radiation was eliminated using the secondary monochromator at the diffracted beam. The scanning electron microscope (SEM), model: Quanta 250, with high-resolution field emission gun (HRFEG, Czech), and high-resolution transmission electron microscope (HR-TEM), model: JEM2100, Japan, were used to study the morphology and particle size and shape of the prepared fertilizers. The adsorption-desorption isotherm of purified N2 at 77 K was carried out using the BELSORP-mini apparatus (BEL Japan, Inc.) that allowed prior outgassing to a residual pressure of 10−5 Torr at 100°C overnight to remove all moisture adsorbed on sample surface and pores.
2.3. The Agriculture Processes
This study is aimed at assessing the effect of micronutrient oxide nanoparticles (zinc, iron, and manganese) as well as their combinations (zinc+iron, zinc+manganese, iron+manganese, and iron+manganese+zinc) as a foliar application on the growth, yield, and quality of squash plants.
2.3.1. Experiment Planning
Seeds of squash (cv. Eskandarani F1) were provided from the Agricultural Research Centre, Ministry of Agricultural and Land Reclamation. Seeds were sown on March 1st in clay soil in Shebin El-Kom, El-Monifia governorate, Egypt, during two seasons 2017 and 2018 and then sown at a rate of one seed per hill and 50 cm distance between the hills on one side of the ridge .
2.3.2. Experiment Treatments
Squash plants were sprayed with zinc, iron, manganese, zinc+iron, zinc+manganese, iron+manganese, and iron+manganese+zinc in the form of oxide nanoparticles with a concentration of 20 ppm for all treatments as compared to control. The experiment was set in a complete randomized block design with three replicates for each particular treatment.
Squash plants were sprayed with treatments after 20 days from the sowing of the seeds. The fertilization, irrigation, and resistance to weeds and diseases of squash plants were carried out according to the recommendations of the Ministry of Agriculture.
2.3.3. Data Recorded
Five plants of squash plants were randomly taken from each experimental plot after 35 days from planting the seeds for measuring the vegetative growth parameters as expressed as plant length, the number of leaves/plant, leave area/plant, and fresh and dry weight of the whole plant. The plants were harvested to determine the fruit length, fruit diameter, yield/plant, and the yield (t/ha). The fruits of the squash plants were collected for a month after 40 days from sowing.
2.3.4. Chemical Analyses
Fresh samples of squash (leaves and fruits) were dried in an oven at 60°C till constant weight, and then dried sample was taken for proximate analysis (100 g dry weight basis) and mineral determination. The following chemical analyses were determined:
(1) Photosynthetic Pigments. Photosynthetic pigments were determined in fresh leaves (35 days from sowing) by a spectrophotometer according to Lichtenthaler and Wellburn .
(2) Proximate Analysis. Organic matter, carbohydrates, protein, lipids, ash, and fiber were determined according to AOAC [24, 26]. The energy value was calculated using the water factor method () .
(3) Mineral Determination. The minerals were determined according to the method described in the AOAC . Where nitrogen was determined by Kjeldahl-N, phosphorus was determined by a spectrophotometer, while the iron, zinc, and manganese were analyzed by atomic absorption, and potassium was determined by a flame spectrophotometer.
2.4. Statistical Analysis
All data were subjected to statistical analysis according to the procedures reported Kobata et al. . The data obtained were subjected to analysis of variance (ANOVA) and analyzed for statistically significant differences using the LSD test at a 5% level.
3. Results and Discussion
3.1. The Nanofertilizer Characterization
3.1.1. X-Ray Diffraction
The crystal structure of the prepared nanooxides was investigated using X-ray diffraction measurements, and the results are shown in Figure 1. The prepared iron oxide nanoparticles showed the patterns corresponding to Fe2O3  such as (012), (104), and (110), as shown in Figure 1(a). On the other hand, the manganese XRD patterns displayed in Figure 1(b) matched well with tetragonal hausmannite demonstrating the successful preparation of Mn3O4 . Figure 1(c) is showing the XRD patterns of the prepared zinc oxide. All the obtained crystal patterns matched the patterns corresponding to ZnO. The XRD patterns of all the samples confirm the purity of all prepared oxides, and no other impurities were detected .
The XRD study proves the successful preparation of pure Fe2O3, Mn3O4, and ZnO as nanoparticles. The average crystallite size of such materials was calculated using the Scherrer equation. The obtained crystallite size was 51.6, 56.0, and 36.8 nm for Fe2O3, Mn3O4, and ZnO, respectively. The values indicated that all the prepared materials were in the nanoscale range.
3.1.2. Oxide Nanoparticle Morphology and Textural Analysis
The transmission and scanning electron microscope diagrams of the prepared nanooxides are shown in Figures 2 and 3, respectively. The average particle size of the prepared nanooxides was around 20-60 nm. Although the materials showed a large particle size, it is still inside the nanorange below 100 nm. Such large size can be contributed to the absence of stabilizers during the synthesis process. This absence was necessary to avoid introducing any foreign component contaminated with the prepared nanooxides during the agriculture process.
The TEM diagrams of Fe2O3 and Mn3O4 nanoparticles showed a uniform diamond shape of such oxides as shown in Figures 2(a) and 2(b), respectively. However, the regularity in particle shape in case of Fe2O3 is clearly observed and much improved than in Mn3O4. This observation is supported by SEM images of Fe2O3 and Mn3O4 as illustrated in Figures 3(a) and 3(b), respectively, where the particles of F2O3 are aggregated forming a clear parallelepiped rectangle structure than in the case of Mn3O4.
On the other hand, the TEM image of ZnO nanoparticles exhibited a small size compared to the other nanoparticles; however, ZnO nanoparticles showed less uniform crystal shapes (Figure 2(c)). Moreover, the SEM image of ZnO (Figure 3(c)) reveals the ingathering of these small size particles constructing an enhanced close packing texture compared to other oxides.
3.1.3. Surface Area and Pore Structure Analysis of Prepared Oxide Nanoparticles
The main surface and pore structure characteristics of the synthesized nanooxides were studied using nitrogen gas adsorption at liquid nitrogen temperature (77 K), and the results are summarized in Table 1. The adsorption-desorption isotherms for all oxide nanoparticles exhibit type II according to the classification of Brunauer-Deming-Deming-Teller [32, 33], with an H3 hysteresis loop [33, 34] as shown in Figure 4. This type of hysteresis arises from the assemblage of loosely coherent particles of a plate-like form giving a rise to slit-shaped pores. Besides, the closure of the hysteresis loops at a low value (<0.4) indicates the presence of some pores of barely accessible entrances .
As expected, ZnO nanoparticles showed the highest surface area due to its small particle size compared to the two other nanooxides. Moreover, the small surface area of Fe2O3 nanoparticles could be due to the high holding temperature during the microwave synthesis process which caused the larger particle size as well as the sintering of the surface-active sites.
3.2. The Squash Planting Process
3.2.1. Effect of Nanooxides on Vegetative Growth Characters of Squash Plant
Data presented in Table 2 indicated that the oxide nanoparticles recorded a significant increase in vegetative growth characters of the squash plant. Plant length, number of leaves per plant, leaf area per plant, and plant fresh weight of squash plant were significantly increased by spraying plants with Mn nanooxide as compared to other treatments. On the contrary, plant dry weight is enhanced with Fe nanooxide and followed by Fe+Mn nanooxides. These results were well held in the two experimental seasons. These results might be due to the function of manganese. The manganese plays a vital role in chlorophyll synthesis, and its occurrence is important in photosystem II, also involved in cell division and plant development [36–38].
3.2.2. Effect of Nanooxides on Vegetative Growth and Yield Characters of Squash Plant
Results in Table 3 revealed that the best fruit length of squash plant is recorded with plants treated with Fe nanooxides compared with other treatments. On the other hand, fruit diameter and yield (kg/plant and t/ha) of fruit squash were significantly affected with Mn nanooxides during two successive seasons 2017 and 2018. At a significant level of 5%, all parameters showed significance, except for fruit diameter which was not significant. This result is due to the better results for vegetative growth with manganese as described in this study in Table 2 and also may be due to enhanced plant nutrition and mounting photosynthesis in squash plants; thus, yield and quality of squash plants may enhance by increasing photosynthetic efficiency . These results were in harmony with those reported by  on potato, [40, 41] on potato,  on crop production, and  on pumpkin.
N.S = not significant ().
3.2.3. Effect of Nanooxides on Photosynthetic Pigments of Squash Leaves
Data recorded in Figure 5 showed clearly that the nanooxides as a foliar application increased the content of photosynthetic pigments expressed as chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in squash leaves as compared to control. The application of manganese nanooxide as foliar on squash plants enhanced the content of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in the leaves followed by plants supplied with iron and zinc nanooxides compared with other treatments, during the seasonal study. This result might be attributed to the function of manganese in the process of photosynthesis, which turns the light of the sun into plant energy, which affects the formation of green plastids in the leaves and the production of chlorophyll [38, 43].
3.2.4. Effect of Nanooxides on Proximate Components of Leaves and Fruits of Squash Plant
Data presented in Table 4 showed that the nanofertilizers foliar fertilizer significantly increased the proximate components of the leaves and fruits of squash plant, during two seasons 2017 and 2018. The content of organic matter, protein, and total energy recorded the higher levels in the leaves of squash plants that had been sprayed with manganese nanooxide. These results might be due to manganese which is used in plants as the main contributor to a variety of biological systems including photosynthesis, respiration, and nitrogen assimilation . While the highest ash content percentage was obtained by using iron nanooxide, as for the fiber content in the leaves, it showed the best ratio with the treatment of iron+manganese nanooxides. Lipid ratio showed the best value in the leaves with plants treated with zinc+iron nanooxides. On the other hand, traditional agriculture (control) recorded the highest percentage of the carbohydrate content in leaves. This result might be due to the interaction between minerals and their effect on the metabolite translocation from the leaves to the other organs of the plant .
Data in Table 4 showed that the content of proximate components of squash fruits was affected by types of nanooxides. The highest value of carbohydrate and total energy is recorded with control in squash fruits. On the contrary, iron nanooxide showed the best percentage of organic matter, protein, and lipid in the fruits compared with other treatments. These results may be due to iron which is involved in protein metabolism in plants . The fiber content in fruits was significantly increased by plants supplied with Zn+Mn nanooxides. On the other hand, the percentage of ash showed the best value with Fe+Mn nanooxides. This result may be due to manganese function with other minerals zinc and iron .
3.2.5. Effect of Nanooxides on Mineral Content of Leaves and Fruits of Squash Plant
Data in Table 5 indicated that the nanooxides significantly increased the endogenous minerals in the leaves and fruits of squash plant during the two seasons of study. Nitrogen percentage appeared increment in squash leaves through plants spraying with manganese nanooxide compared to other nanooxide. This effect might be due to the relations between the different ions of the minerals and their translocation from the leaves to the fruits. The best content of manganese and iron in squash leaves showed with plants spraying with zinc nanooxide. This result might be due to manganese and iron concentrations enhanced significantly with the zinc application . Zinc is led to iron transfer from root to shoot [45, 46]. While the squash plants, which were treated by Zn+Mn nanooxide, recorded the maximum percentage of phosphorus and potassium in leaves, iron+manganese nanooxide gave the highest value of zinc content in leaves. These results might be due to manganese interaction with other elements .
The effect of different nanooxides on the mineral content of squash fruit is presented in Table 5, spraying nanooxides on squash plants had a significant effect on squash fruit content of the elements. The iron nanooxide showed a significant effect on the content of nitrogen in the fruit of squash plants. The highest value of phosphorus was recorded with zinc+manganese nanooxides. In this trend, Fe+Mn nanooxides enhanced the content of potassium and iron in fruits, while Zn+Fe nanooxides improved the content of manganese in fruits. The maximum content of zinc in fruits appeared with Fe+Mn+Zn nanooxides. These results might be due to the role of the elements in metabolic processes and penetration to the plant cell, and also, manganese function is closely related to iron . Also, Roosta et al.  showed that the nano-Fe-chelate has a valuable result on the content of manganese and zinc in lettuce plant. The trend of obtained results is in good accordance with Schmidt et al. , who found that manganese is an important micronutrient with an indispensable role as a catalyst in the oxygen-evolving complex of photosystem, respiration, and nitrogen assimilation. It is necessary by plants in the second greatest quantity compared with ferric. Thus, manganese competes with the micronutrients (Fe, Zn, Cu, Mg, and Ca) for uptake by the plant.
The privilege of using such nutrients in the nanoscale is to increase its ability towards diffusion through the pores of the plant cell. In addition, the mutagenic can occur in the plant by these nanomaterials improving its growth and yield [11, 49].
Zinc, manganese, and iron nanooxides have been successfully synthesized via a green chemistry technique using a microwave-assisted hydrothermal method. The resulted nanooxides have an average particle size around 20-60 nm and small surface areas. These oxides were applied as a foliar nanofertilizer on squash plants. The use of the prepared nanooxides as a foliar application improved the growth and the yield in comparison with untreated plants. Furthermore, the yield (kg/plant and tons/hectare) of fruit squash was significantly affected with Mn nanooxide especially when it is used individually or combined with Fe nanooxide. Also, the content of organic matter, protein, lipids, and energy recorded the higher levels in fruits of squash plants that have been sprayed with Fe nanooxide.
The data used to support the findings of this study are available from the corresponding author upon request.
Conflicts of Interest
The authors declare that they have no competing interests.
This work was supported by the PhosAgro/UNESCO/IUPAC partnership in green chemistry for life, grant number: 4500333878-A1.
- M. Rai and A. Ingle, “Role of nanotechnology in agriculture with special reference to management of insect pests,” Applied Microbiology and Biotechnology, vol. 94, no. 2, pp. 287–293, 2012.
- B. Zhang, B. A. Pinsky, J. S. Ananta et al., “Diagnosis of Zika virus infection on a nanotechnology platform,” Nature Medicine, vol. 23, no. 5, pp. 548–550, 2017.
- D. Rawtani, N. Khatri, S. Tyagi, and G. Pandey, “Nanotechnology-based recent approaches for sensing and remediation of pesticides,” Journal of Environmental Management, vol. 206, pp. 749–762, 2018.
- D.-Y. Kim, A. Kadam, S. Shinde, R. G. Saratale, J. Patra, and G. Ghodake, “Recent developments in nanotechnology transforming the agricultural sector: a transition replete with opportunities,” Journal of the Science of Food and Agriculture, vol. 98, no. 3, pp. 849–864, 2018.
- R. Prasad, A. Bhattacharyya, and Q. D. Nguyen, “Nanotechnology in sustainable agriculture: recent developments, challenges, and perspectives,” Frontiers in Microbiology, vol. 8, pp. 1–13, 2017.
- I. Iavicoli, V. Leso, D. H. Beezhold, and A. A. Shvedova, “Nanotechnology in agriculture: opportunities, toxicological implications, and occupational risks,” Toxicology and Applied Pharmacology, vol. 329, pp. 96–111, 2017.
- M. Sharon, A. K. Choudhary, and R. Kumar, “Nanotechnology in agricultural diseases and food safety,” Journal of Phytology, vol. 2, no. 4, pp. 83–92, 2010.
- P. Zuo, X. Li, D. C. Dominguez, and B.-C. Ye, “A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection,” Lab on a Chip, vol. 13, no. 19, pp. 3921–3928, 2013.
- V. Ghormade, M. V. Deshpande, and K. M. Paknikar, “Perspectives for nano-biotechnology enabled protection and nutrition of plants,” Biotechnology Advances, vol. 29, no. 6, pp. 792–803, 2011.
- K. S. Subramanian, A. Manikandan, M. Thirunavukkarasu, and C. S. Rahale, “Nano-fertilizers for balanced crop nutrition,” in Nanotechnologies in Food and Agriculture, M. Rai, C. Ribeiro, L. Mattoso and N. Duran, Ed., pp. 69–80, Springer International Publishing, Cham, 2015.
- D. M. Salama, S. A. Osman, M. E. Abd El-Aziz, M. S. A. Abd Elwahed, and E. A. Shaaban, “Effect of zinc oxide nanoparticles on the growth, genomic DNA, production and the quality of common dry bean (Phaseolus vulgaris),” Biocatalysis and Agricultural Biotechnology, vol. 18, article 101083, 2019.
- T. Munir, M. Rizwan, M. Kashif et al., “Effect of zinc oxide nanoparticles on the growth and Zn uptake in wheat (Triticum aestivum L.) by seed priming method,” Digest Journal of Nanomaterials & Biostructures (DJNB), vol. 13, no. 1, 2018.
- M. V. Khodakovskaya, B. S. Kim, J. N. Kim et al., “Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community,” Small, vol. 9, no. 1, pp. 115–123, 2013.
- W. Du, J. Yang, Q. Peng, X. Liang, and H. Mao, “Comparison study of zinc nanoparticles and zinc sulphate on wheat growth: from toxicity and zinc biofortification,” Chemosphere, vol. 227, pp. 109–116, 2019.
- E. E. Shaban, H. F. H. Elbakry, K. S. Ibrahim, E. M. El Sayed, D. M. Salama, and A.-R. H. Farrag, “The effect of white kidney bean fertilized with nano-zinc on nutritional and biochemical aspects in rats,” Biotechnology Reports, vol. 23, article e00357, 2019.
- M. Rui, C. Ma, Y. Hao et al., “Iron oxide nanoparticles as a potential iron fertilizer for peanut (Arachis hypogaea),” Frontiers in Plant Science, vol. 7, pp. 815–824, 2016.
- J. Novillo, M. I. Rico, and J. M. Alvarez, “Controlled release of manganese into water from coated experimental fertilizers. Laboratory characterization,” Journal of Agricultural and Food Chemistry, vol. 49, no. 3, pp. 1298–1303, 2001.
- I. Bhattacharya, S. Bandyopadhyay, C. Varadachari, and K. Ghosh, “Development of a novel slow-releasing iron− manganese fertilizer compound,” Industrial & Engineering Chemistry Research, vol. 46, no. 9, pp. 2870–2876, 2007.
- S. Bandyopadhyay, K. Ghosh, and C. Varadachari, “Multimicronutrient slow-release fertilizer of zinc, iron, manganese, and copper,” International Journal of Chemical Engineering, vol. 2014, 7 pages, 2014.
- X. a. Fan, J. Guan, W. Wang, and G. Tong, “Morphology evolution, magnetic and microwave absorption properties of nano/submicrometre iron particles obtained at different reduced temperatures,” Journal of Physics D: Applied Physics, vol. 42, no. 7, pp. 075006–075013, 2009.
- C.-H. Lu, B. Bhattacharjee, and S.-Y. Chen, “Microwave synthesis of manganese-ion-doped zinc sulfide nano-phosphors using a novel monomer,” Journal of Alloys and Compounds, vol. 475, no. 1-2, pp. 116–121, 2009.
- P. Parhi, J. Kramer, and V. Manivannan, “Microwave initiated hydrothermal synthesis of nano-sized complex fluorides, KMF3 (K = Zn, Mn, Co, and Fe),” Journal of Materials Science, vol. 43, no. 16, pp. 5540–5545, 2008.
- Y. Zheng, J. Tan, L. Huang et al., “Sonochemistry-assisted microwave synthesis of nano-sized lanthanide activated phosphors with luminescence and different microstructures,” Materials Letters, vol. 113, pp. 90–92, 2013.
- P. Feldsine, C. Abeyta, and W. H. Andrews, “AOAC International Methods Committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis,” Journal of AOAC International, vol. 85, no. 5, pp. 1187–1200, 2002.
- H. K. Lichtenthaler and A. R. Wellburn, “Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents,” Biochemical Society Transactions, vol. 11, no. 5, pp. 591-592, 1983.
- M. E. Abd El-Aziz, S. M. M. Morsi, D. M. Salama et al., “Preparation and characterization of chitosan/polyacrylic acid/copper nanocomposites and their impact on onion production,” International Journal of Biological Macromolecules, vol. 123, pp. 856–865, 2019.
- T. U. Nwabueze, “Nitrogen solubility index and amino acid profile of extruded African breadfruit (T. africana) blends,” Nigerian Food Journal, vol. 25, no. 2, pp. 23–35, 2007.
- T. Kobata, M. Koç, C. Barutçular, K.-i. Tanno, and M. Inagaki, “Harvest index is a critical factor influencing the grain yield of diverse wheat species under rain-fed conditions in the Mediterranean zone of southeastern Turkey and northern Syria,” Plant Production Science, vol. 21, no. 2, pp. 71–82, 2018.
- M. Heikal, “Characteristics, textural properties and fire resistance of cement pastes containing Fe2O3 nano-particles,” Journal of Thermal Analysis and Calorimetry, vol. 126, no. 3, pp. 1077–1087, 2016.
- Y. Qiao, Q. Sun, O. Sha et al., “Synthesis of Mn3O4 nano-materials via CTAB/SDS vesicle templating for high performance supercapacitors,” Materials Letters, vol. 210, pp. 128–132, 2018.
- R. Kumar, G. Kumar, M. S. Akhtar, and A. Umar, “Sonophotocatalytic degradation of methyl orange using ZnO nano-aggregates,” Journal of Alloys and Compounds, vol. 629, pp. 167–172, 2015.
- S. Brunauer, L. S. Deming, W. E. Deming, and E. Teller, “On a theory of the van der Waals adsorption of gases,” Journal of the American Chemical Society, vol. 62, no. 7, pp. 1723–1732, 1940.
- S. J. Gregg, K. S. W. Sing, and R. Haul, “Adsorption, Surface Area and Porosity. 2. Auflage, academic press, London,” Berichte der Bunsengesellschaft für Physikalische Chemie, vol. 86, no. 10, pp. 957–957, 1982.
- P. T. Tanev and L. T. Vlaev, “An attempt at a more precise evaluation of the approach to mesopore size distribution calculations depending on the degree of pore blocking,” Journal of Colloid and Interface Science, vol. 160, no. 1, pp. 110–116, 1993.
- G. M. El Shafei, N. A. Moussa, and Z. A. Omran, “Titania-induced changes of silica texture upon moderate heating,” Powder Technology, vol. 107, no. 1-2, pp. 118–122, 2000.
- J. A. A. Ortiz-Villajos, F. J. G. Navarro, C. J. S. Jiménez, C. P. de los Reyes, R. G. Moreno, and R. J. Ballesta, “Trace elements distribution in red soils under semiarid Mediterranean environment,” International Journal of Geosciences, vol. 02, no. 02, pp. 84–97, 2011.
- S. R. Mousavi, M. Shahsavari, and M. Rezaei, “A general overview on manganese (Mn) importance for crops production,” Australian Journal of Basic and Applied Sciences, vol. 5, no. 9, pp. 1799–1803, 2011.
- S. B. Schmidt, P. E. Jensen, and S. Husted, “Manganese deficiency in plants: the impact on photosystem II,” Trends in Plant Science, vol. 21, no. 7, pp. 622–632, 2016.
- K. Kelling, P. Speth, and Department of Soil Science, University of Wisconsin–Madison, Effect of Micronutrient on Potato Tuber Yield and Quality at Spooner, 2001.
- C. Guozhong, Nanostructures and Nanomaterials: Synthesis, Properties and Applications, World Scientific, 2004.
- S. R. Mousavi, M. Galavi, and G. Ahmadvand, “Effect of zinc and manganese foliar application on yield, quality and enrichment on potato (Solanum tuberosum L.),” Asian Journal of Plant Sciences, vol. 6, no. 8, pp. 1256–1260, 2007.
- M. Yousefi and P. Zandi, “Effect of foliar application of zinc and manganese on yield of pumpkin (Cucurbita pepo L.) under two irrigation patterns,” Electronic Journal of Polish Agricultural Universities. Series Agronomy, vol. 15, no. 4, pp. 1–9, 2012.
- R. Millaleo, M. Reyes-Díaz, A. Ivanov, M. Mora, and M. Alberdi, “Manganese as essential and toxic element for plants: transport, accumulation and resistance mechanisms,” Journal of Soil Science and Plant Nutrition, vol. 10, no. 4, pp. 470–481, 2010.
- G. R. Rout and S. Sahoo, “Role of iron in plant growth and metabolism,” Reviews in Agricultural Science, vol. 3, no. 0, pp. 1–24, 2015.
- Z. Rengel and V. Römheld, “Root exudation and Fe uptake and transport in wheat genotypes differing in tolerance to Zn deficiency,” Plant and Soil, vol. 222, no. 1/2, pp. 25–34, 2000.
- Z. Rengel, V. Römheld, and H. Marschner, “Uptake of zinc and iron by wheat genotypes differing in tolerance to zinc deficiency,” Journal of Plant Physiology, vol. 152, no. 4-5, pp. 433–438, 1998.
- S. K. Das, “Role of micronutrient in rice cultivation and management strategy in organic agriculture—a reappraisal,” Agricultural Sciences, vol. 05, no. 09, pp. 765–769, 2014.
- H. R. Roosta, M. Jalali, and S. M. Ali Vakili Shahrbabaki, “Effect of nano Fe-chelate, Fe-Eddha and FeSO4 on vegetative growth, physiological parameters and some nutrient elements concentrations of four varieties of lettuce (Lactuca sativa L.) in NFT system,” Journal of Plant Nutrition, vol. 38, no. 14, pp. 2176–2184, 2015.
- F. Aslani, S. Bagheri, N. Muhd Julkapli, A. S. Juraimi, F. S. G. Hashemi, and A. Baghdadi, “Effects of engineered nanomaterials on plants growth: an overview,” The Scientific World Journal, vol. 2014, 28 pages, 2014.
Copyright © 2019 Ahmed Shebl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.