Simple Label-Free Electrochemical Immunosensor in a Microchamber for Detecting Newcastle Disease Virus

Table 2

Comparison of analytical properties of different methods for the detection of NDV.

Technique	Immobilization	Type	Antibody	Virus	Limit of detection	Ref.
Optic	APTES/CDI	Indirect	Goat monoclonal IgY	Purified NDV	2 ng/mL	[30]
RT-PCR				Inactivated NDV	5 × 10^2 ELD50/mL	[31]
RT-PCR				Inactivated NDV	5 × 10^2 ELD50/mL	[5]
RT-PCR				Inactivated NDV	10^5.8 ELD50/mL	[6]
RRT-PCR				NDV RNA	10 EID50/mL	[1]
CV	PrA/GA	Direct	IgY	Inactivated NDV	9 EID50/mL	This work
CV	SAM/NHS	Direct	IgY	Inactivated NDV	5 EID50/mL

NDV: Newcastle disease virus; EID: embryo infectious dose; ELD: embryo lethal dose; RT-PCR: reverse transcriptase-polymerase chain reaction; RRT-PCR: real-time reverse transcription PCR.