Analysis of the Immune and Antioxidant Response of Cellulose Nanocrystals Grafted with β-Cyclodextrin in Myeloid Cell Lines

Figure 2

Immunological response of human and mouse cells treated with cellulose nanocrystal-β-cyclodextrin (CNCs-β-CD), in the presence or absence of lipopolysaccharide (LPS, 100 ng/mL). THP-1 cell lines were initially incubated with phorbol 12-myristate 13-acetate (PMA, 3 ng/mL) for 24 h and then concomitant with LPS (100 ng/mL) for 4-5 h followed by the addition of CNCs-β-CD (10–50 μg/mL) for another 19-20 h. Secreted (a) IL-1β by THP-1 was quantified in the cell supernatants by ELISA. Alternatively, mouse macrophage-like cells (J774A.1) were treated with LPS (100 ng/mL) for 4-5 h followed by the addition of CNCs-β-CD (25 and 50 μg/mL) for another 19-20 h. Secreted (b) IL-1β and (c) TNFα by J774A.1 were quantified in the cell supernatants by ELISA. Data were means ± SD from triplicate. compared to respective control.

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