Figure 5: Discriminant function analysis of the biomarker responses. Principal component (a) and discriminant function (b) analyses were performed with biomarker data from mussels exposed to 50 μg/L nAg with 4 different coatings: citrate, PVP, silicate, and bPEI. For principal component analysis, the total variance was explained at 80%. dg: digestive gland; g: gills; GSI: gonadosomatic index; HIS: hepatosomatic index; CF: condition factor. For discriminant function analysis, the mean classification was at 83% and the total explained variance was 80%.