Exosome: A Novel Nanocarrier Delivering Noncoding RNA for Bone Tissue Engineering

Figure 3

Characterization and cellular uptake of exosomes. As shown in (a) and (b), the morphology of exosomes was observed under TEM and SEM. In (c) and (d), exosome is a round membranous vesicle with a diameter of about 30-150 nm and the results of western blot showed that exosome was able to express marker proteins CD9, CD81, and CD63. In (e), to verify whether exosome interacts with cell, exosome labeled by Dil and receptor cell were cocultured in vitro for 6 h. Under a fluorescence microscope, over 85% of cell showed positive result of Dil, indicating that the Dil-labeled exosome has been efficiently transferred to receptor cells [30, 35, 53]. (a–d) Copyright 2020 BBRC published by Elsevier Ltd. (b) Copyright 2019 EXCLI J published by University of Mainz. (c) Copyright 2020 Int Immunopharmacology published by Elsevier Ltd.

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