Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells

Figure 5

Graphene films enhanced neural-differentiation of hNSCs: (a) Bright-field images of hNSCs that were differentiated for three days (left), two weeks (middle), and three weeks (right). (b) Bright-field (top row) and fluorescence (bottom row) images of hNSCs that were differentiated on glass (left) and graphene (right) for one month. TUJ1 (green) was used for the immunostaining of neural cells. GFAP (red) was for astroglial cells, and DAPI (blue) was for nuclei. (c) After differentiation for one month, the numbers of cells per 0.64 mm² were counted for the graphene and glass regions. (d) Percentages of immunoreactive cells for TUJ1 (green) and GFAP (red) on glass and graphene. All scale bars represent 200 μm. The figure was reproduced from Ref [114] with permission.

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