In Vivo Evaluation of the Antitumor and Immunogenic Properties of Silver and Sodium Dichloroacetate Combination against Melanoma

Figure 1

Cell viability of B16F10 cells. B16F10 cells were cultured in 96-well plates ( cells/well) at 37°C and 5% CO₂ for 24 hours. Then, cells were treated with Ag ( mM), DCA (0-300 mM), or ( mM and 0-195 mM, respectively) for 4 hours. Cell viability was determined with the resazurin assay; briefly, treatments were removed, and cells were incubated with the resazurin reagent (20% ) for 1 hour; past this time, fluorescence of converted resorufin was determined at 530nm excitation wavelength and 590 emission wavelength. Increased fluorescent signal was quantified as cell viability. Graph bars show the mean of three independent . Statistical difference () was determined with the Dunnett post hoc test. There is statistical difference between the control and labelled bars in the graph ().

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