Research Article
In Vivo Evaluation of the Antitumor and Immunogenic Properties of Silver and Sodium Dichloroacetate Combination against Melanoma
Figure 4
Calreticulin surface exposure in B16F10 cells. B16F10 cells were cultured in 24-well plates ( cells/well) at 37°C and 5% CO2 for 24 hours. Then, cells were treated with Ag (mM), DCA (19mM), or (mM and 135mM, respectively) for 4 hours. Calreticulin exposure was determined by flow cytometry. The figure shows (a) size versus granularity representative dot plots of the analyzed cell population (above) and cell count versus stain intensity representative histograms. (b) Positive stain average of three independent experiments. There is no statistical difference () between bars as determined by one-way ANOVA and the Tukey post hoc test.
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