In Vivo Evaluation of the Antitumor and Immunogenic Properties of Silver and Sodium Dichloroacetate Combination against Melanoma

Figure 5

Tumor implantation in vaccinated mice. C57BL/6 mice were randomly divided into 4 groups: mice without immunization (control) and mice immunized with B16F10 cells () lysed with Ag ( mM), DCA (300 mM), or (mM and 195mM, respectively). Seven days after immunization, mice were challenged with B16F10 viable cells injected into the left flank. Tumor appearance was monitored daily by palpation, and on the day of the sacrifice, tumor volume was determined with the equation: , where is the longest side and is the shortest side. The image shows the percentage of mice without tumor (tumor free mice) (a), tumor volume (b), and weight (c) on day 12 after the challenge, and a representative mouse, tumor, and lungs of each group. (d) There is no statistical difference () between bars labelled with the () in the graph.

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