A Unique Protein Self-Assembling Nanoparticle with Significant Advantages in Vaccine Development and Production

Figure 8

Engineered gD-NSP (where NSP denotes the NSP10 sequence 9-129) fusion protein sequences. (a) NSP10 sequence as incorporated in pcDNA3.1 vector (following DNA optimization for expression in humans). Note: the native HVS gD transcription leader sequence is retained and there are no His-tags in this design. (b) NSP10 final sequence for incorporation in pET17b for expression in bacteria (following DNA optimization for expression in E. coli). Note: the viral transcription leader sequence, irrelevant in a prokaryotic expression system, has been removed and a C-terminal His-tag added. Red highlighted sequences denote linker and His-tag sequences.

(a)

(b)