Journal of Nanomaterials / 2020 / Article / Fig 2

Research Article

Sequential Delivery of BMP2-Derived Peptide P24 by Thiolated Chitosan/Calcium Carbonate Composite Microspheres Scaffolds for Bone Regeneration

Figure 2

(a) CCK-8 test of BMSCs viability after coculture with TCS/CA, TCS-5%P24/CA, and TCS-10%P24/CA scaffolds at days 1, 3, 7, and 10. (b) The relative ALP activity of BMSCs cultured in a scaffold-stimulated medium. (c) Alizarin red S staining of BMSCs cultured with TCS/CA, TCS-5%P24/CA, and TCS-10%P24/CA scaffolds for 21 days. (d) The level of the mRNA for osteogenic-specific genes (Runx2 and ALP) and related matrix genes (COL1a1) of rat BMSCs cultured on TCS-P24/CA scaffolds for 7 days. Levels, quantified using real-time RT-PCR, are normalized to the reference gene GAPDH. (; ); Error bars represent ().

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