Characterization of the obtained copolymers. (a) 1H NMR spectrum of Gln-PEG-b-PAE copolymer. (b) The critical micelle concentration (CMC) of Gln-PEG-b-PAE copolymer is measured by the intensity ratio of the first emission peak to the third emission peak I1/I3 (the fluorescence intensity at 373 nm and at 384 nm). (c) TEM photo of Gln-PEG-b-PAE@DOX micelles. Hydrodynamic size distribution profiles of (d) Gln-PEG-b-PAE copolymer and (e) Gln-PEG-b-PAE@DOX micelles determined by dynamic light scattering (DLS).