A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

Figure 1

Design of the novel LSPR biosensor for detection of HE4. (a). Glass substrate. (b). Triangle-shaped silver nanoparticles array synthesized on glass substrate. (c). Incubation of 1 mM MUA. (d). Incubated in 75 mM EDC-HCl/15 mM NHS. (e). Incubated with 100ug/ml protein G. (f). Immobilization of anti-HE4 monoclonal antibodies (10 μg/mL). (g). Immunoassay of HE4 in buffer or urine samples.