A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed

<div>LSPR spectra for biosensor design and detection of 1 pM HE4. (a) Bare silver nanochip, <i>λ</i>max =606.16 nm. (b) Modification of 1 mM MUA, <i>λ</i>max =619.12 nm. (c) Incubation with 100ug/ml protein G, <i>λ</i>max =624.79 nm. (d) Immobilization of 10 <i>μ</i>g/mL anti-HE4 monoclonal antibodies, <i>λ</i>max =633.48 nm. (e) Detection of 1 pM HE4, <i>λ</i>max =639.35 nm. Note: All spectra were collected at room temperature in air.</div>

Journal of Nanomaterials

fig3

Figure 3

Figure 3: A Novel Label-Free Biosensor for Detection of HE4 in Urine Based on Localized Surface Plasmon Resonance and Protein G Directional Fixed 