miRNA-146a and miRNA-202-3p Attenuate Inflammatory Response by Inhibiting TLR4, IRAK1, and TRAF6 Expressions in Rats following Spinal Cord Injury

Figure 4

The mRNA and protein expressions of TLR4, IRAK1, and TRAF6 in rats following SCI. (a) qRT-PCR analysis of TLR4, IRAK1, and TRAF6 mRNA expressions in sham-operated and SCI rats at days 1, 3, and 7 after surgery. (b) qRT-PCR analysis of TLR4, IRAK1, and TRAF6 mRNA expressions among SCI, miRNA-146a mimic, and miRNA-146a antagomir groups at days 1, 3, and 7 after surgery. (c) qRT-PCR analysis of TLR4, IRAK1, and TRAF6 mRNA expressions among SCI, miRNA-202-3p mimic, and miRNA-202-3p antagomir groups at days 1, 3, and 7 after surgery. (d) Immunoblotting analysis of TLR4, IRAK1, and TRAF6 protein expressions in sham-operated and SCI rats at day 7 after surgery. (e) Immunoblotting analysis of TLR4, IRAK1, and TRAF6 protein expressions among SCI, miRNA-146a mimic, and miRNA-146a antagomir groups at day 7 after surgery. (f) Immunoblotting analysis of TLR4, IRAK1, and TRAF6 protein expressions among SCI, miRNA-202-3p mimic, and miRNA-202-3p antagomir groups at day 7 after surgery. by Student’s -test in (a) and (d); by one-way ANOVA with Tukey’s test in other panels.

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