miRNA-146a and miRNA-202-3p Attenuate Inflammatory Response by Inhibiting TLR4, IRAK1, and TRAF6 Expressions in Rats following Spinal Cord Injury

Figure 6

miRNA-146a and miRNA-202-3p inhibited the release of inflammatory cytokines in LPS-stimulated PC12 cells by regulating TLR4, IRAK1, and TRAF6. (a) Immunoblotting analysis of TLR4, IRAK1, and TRAF6 proteins in PC12 cells transfected with si-TLR4, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. (b) ELISA detection of TNF-α, IL-1β, IL-6, and IL-8 levels in PC12 cells transfected with si-TLR4, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. (c) Immunoblotting analysis of IRAK1 and TRAF6 proteins in PC12 cells transfected with si-IRAK1, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. (d) ELISA detection of TNF-α, IL-1β, IL-6, and IL-8 levels in PC12 cells transfected with si-IRAK1, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. (e) Immunoblotting analysis of TRAF6 protein in PC12 cells transfected with si-TRAF6, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. (f) ELISA detection of TNF-α, IL-1β, IL-6, and IL-8 levels in PC12 cells transfected with si-TRAF6, anti-miRNA-146a, anti-miRNA-202-3p, and si-NC. by one-way ANOVA with Tukey’s test.

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