Fentanyl Exerts an Antitumor Effect on Papillary Thyroid Cancer by Regulating the miR-204/KLF5 Axis

Figure 3

KLF5 was the target of miR-204. (a) The results of PCR showed that miR-204 overexpression inhibited the expression of KLF5 at mRNA levels. (b) The results of western blot assays also verified the miR-204 overexpression at protein levels. (c) The sequence of 3UTR of KLF5 contained the potential binding sites for miR-204. (d) The luciferase reporter assay identified that miR-204 directly targeted KLF5. (e) The results of CCK-8 assays indicated that KLF5 knockdown reversed the positive effects on the proliferation of PTC cells induced by miR-204 inhibitor. (f, g) The results of Transwell showed that miR-204 showed its negative effects on the cell invasion via targeting KLF5. Student’s -test was used for statistical analysis in (a, d). Repeated measurement ANOVA with Bonferroni corrections was used in (e). One-way ANOVA was used in (e, g). .

(a)

(b)

(c)

(d)

(e)

(f)

(g)