MicroRNA-34c-5p Inhibition of NUF2 Suppresses Lung Adenocarcinoma Cell Viability and Invasion

Figure 5

miR-34c-3p mediates A549 cells by binding to NUF2. (a) The binding sites of miR-34c-5p and NUF2 are predicted in the RNA22 database (https://cm.jefferson.edu/). (b) Coexpression of miR-34c-5p and NUF2 in lung adenocarcinoma in the starBase database (http://starbase.sysu.edu.cn/). (c) NUF2 as the target gene of miR-34c-3p was verified by luciferase activity assays ( stands for when compared with pmirGLO-NUF2-MUT+miR-34c-5p mimic). (d) NUF2 mRNA and protein expressions in A549 cells were determined by qRT-PCR and Western blot analysis, normalized to GAPDH ( stands for when compared with NC mimic or NC inhibitor). (e) Cell viability was examined by MTT assays. (f) Cell invasion was examined by Transwell assays.

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