Antibacterial Activity of Reduced Graphene Oxide

Figure 5

Cell viability assessment of E. coli upon exposure to (particle-free) RGO leachate. (a) Growth analysis of E. coli when exposed to leachate of increasing RGO concentrations over a time period of 10 h. The respective doubling times are mentioned in the inset. The data represents the average of three biological replicates (different bacterial inocula and leachate preparations), each with two technical replicates (). The error bars represent the standard deviation (SD). (b) Cell viability assessment (3 h, 37°C) using the agar colony counting method. For each leachate system, the data represents the percentages of viable cells at the end of the exposure relative to those at time zero. The data is the average of three biologically independent experiments (different bacterial inocula and leachate preparations), each with three technical replicates (). The error bars represent the standard error of mean (SEM).

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