Attenuation of Liver Injury via Bicyclol–Bovine Serum Albumin Nanopreparation using a Green Synthetic Approach

Figure 1

Scheme of BIC-NPs preparation: (a) 40 mg/ml BSA, SDS (2%), and DTT (0.15%) solution was used for eliminating hydrophobic forces and intramolecular disulfide bonds, we desaturated BSA using SDS and DTT to liberate free thiols, and then the above BSA solution was heated to 90°C for 2 hr to obtain reduced BSA; (b) the treated BSA was redissolved in MES, while BIC-NPs was prepared by adding BIC into reaction solution.

(a)

(b)