miR-622 Induces Breast Cancer Cell MCF-7 Proliferation, Migration, and Invasion by Directly Negatively Targeting EYA1

Figure 3

EYA1 was the direct target of miR-622 in breast cancer. (a, b) through TargetScan, miRDB, and microbase software database screening, we found that EYA1 was a predicted target of miR-622 and that there were two binding sites of miR-622 in the 3UTR of EYA1. (c) miR-622 only affects the wild-type 3UTR of EYA1, but not the mutant-type, as determined by the luciferase assay (EYA1-WT: miR-622 mimic vs. miR-NC, ; EYA1-MT: miR-622 mimic vs. miR-NC, ). (d–f) After transfection of miR-622 mimic, the relative expression levels of EYA1 mRNA and protein in the miR-622 mimic group were lower than those in the miR-NC and control group (EYA1 mRNA: miR-622 mimic vs. miR-NC, ; miR-622 mimic vs. control group, ; EYA1 protein: miR-622 mimic vs. miR-NC, ; miR-622 mimic vs. control group, ).

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