miR-622 Induces Breast Cancer Cell MCF-7 Proliferation, Migration, and Invasion by Directly Negatively Targeting EYA1

Figure 4

Roles of EYA1 in proliferation, migration, and invasion of breast cancer cells. (a–c) After transfection of si-EYA1, the relative expression levels of EYA1 mRNA and protein in the si-EYA1 group were lower than those in the siRNA-NC and control group (EYA1 mRNA: si-EYA1 vs. siRNA-NC, ; si-EYA1 vs. control group, ; EYA1 protein: si-EYA1 vs. siRNA-NC, ; si-EYA1 vs. control group, ). (d) After transfection of si-EYA1 for 48 hours, the proliferation of the si-EYA1 group was stronger than that of the siRNA-NC and control group (si-EYA1 vs. siRNA-NC, ; si-EYA1 vs. control group, ). (e, f) The mobility in the si-EYA1 group () was higher than that in the siRNA-NC group () and the control group () (si-EYA1 vs. siRNA-NC, ; si-EYA1 vs. control group, ). (g, h) The number of invasive cells in the control group and the siRNA-NC group was lower than that in the si-EYA1 group (si-EYA1 vs. siRNA-NC, ; si-EYA1 vs. control group, ).

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