The Aptamer Functionalized Nanocomposite Used for Prostate Cancer Diagnosis and Therapy

Figure 1

Schematic illustration of aptamer selection by systematic evolution of ligands by exponential enrichment (SELEX). The starting single-stranded DNA or RNA library (10¹³~1015random oligonucleotides) is composed of sequences 20~100 nucleotides in length. Each unique sequence contains random bases (30–50 nt) flanked by two conserved primer binding sites, which are used for PCR amplification by annealing primers. After incubation with the target of interest, the bound oligonucleotides are partitioned from unbound sequences. The target-bound sequences are amplified by PCR, and the resulting new subpool is utilized for the next round of selection.