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Journal of Nutrition and Metabolism
Volume 2010, Article ID 287030, 6 pages
Research Article

Milk Products Containing Bioactive Tripeptides Have an Antihypertensive Effect in Double Transgenic Rats (dTGR) Harbouring Human Renin and Human Angiotensinogen Genes

1Institute of Biomedicine, Pharmacology, University of Helsinki, 00014 Helsinki, Finland
2Research Center, Valio Ltd, 00370 Helsinki, Finland
3Medcare Oy, 44100 Äänekoski, Finland
4Max Delbruck Center for Molecular Medicine, 13125 Berlin, Germany
5Foundation for Nutrition Research, 00039 Helsinki, Finland

Received 13 July 2009; Accepted 18 September 2009

Academic Editor: Stanley Omaye

Copyright © 2010 Tiina Jauhiainen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Tripeptides isoleucyl-prolyl-proline (IPP) and valyl-prolyl-proline (VPP) act as ACE inhibitors in vitro. Double transgenic rats (dTGR) harbouring human renin and human angiotensinogen genes develop malignant hypertension due to increased angiotensin II formation. The present study was aimed to evaluate possible antihypertensive effect of IPP and VPP in this severe model. Four-week-old dTGR were randomized in three groups to receive: (1) water (control), (2) fermented milk containing IPP and VPP, and (3) IPP and VPP dissolved in water for three weeks. Fermented milk, but not peptides in water, attenuated the development of hypertension in dTGR by 19 mmHg versus the control group ( ). In vitro vascular function tests showed that high concentrations of the peptides evinced ACE inhibitory properties. In other hypertension related variables, no significant differences between the treatment groups were found. In conclusion, fermented milk product containing IPP and VPP prevents development of malignant hypertension in an animal model.