Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">GENE</td><td align="center">Forward Primer (<svg height="13.175" id="M35" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 24.4375 13.175" width="24.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.175)">
<g transform="translate(72,-61.46)">
<text transform="matrix(1,0,0,-1,-71.95,61.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">5</tspan>
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<text transform="matrix(1,0,0,-1,-65.7,67.01)">
<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
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<text transform="matrix(1,0,0,-1,-64.31,61.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">-</tspan>
<tspan style="font-size: 12.50px; " x="2.088001" y="0">3</tspan>
</text>
<text transform="matrix(1,0,0,-1,-55.98,66.86)">
<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
</text>
</g>
</g>
</svg>)</td><td align="center">Reverse Primer (<svg height="13.175" id="M36" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 24.4375 13.175" width="24.4375" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.175)">
<g transform="translate(72,-61.46)">
<text transform="matrix(1,0,0,-1,-71.95,61.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">5</tspan>
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<text transform="matrix(1,0,0,-1,-65.7,67.01)">
<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
</text>
<text transform="matrix(1,0,0,-1,-64.31,61.69)">
<tspan style="font-size: 12.50px; " x="0" y="0">-</tspan>
<tspan style="font-size: 12.50px; " x="2.088001" y="0">3</tspan>
</text>
<text transform="matrix(1,0,0,-1,-55.98,66.86)">
<tspan style="font-size: 8.75px; " x="0" y="0"></tspan>
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</svg>)</td><td align="center">Accession No.</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left">SREBP1c</td><td align="center">TGCGGACGCAGTCTGGGCAAC</td><td align="center">GTCACTGTCTTGGTTGTTGATG</td><td align="center">AF286469</td></tr><tr><td align="left">SCD-1</td><td align="center">TGGGTTGGCTGCTTGTG</td><td align="center">GTGTGGGCAGGATGAAG</td><td align="center">NM 139192</td></tr><tr><td align="left">FAS</td><td align="center">TGCTCCCAGCTGCAGGC</td><td align="center">GCCCGGTAGCTCTGGGTGTA</td><td align="center">NM 017332</td></tr><tr><td align="left">G3PDH</td><td align="center">GCTTCGGTGACAACACCA</td><td align="center">AGCTGCTCAATGGACTTTCC</td><td align="center">NM 022215</td></tr><tr><td align="left">LEPTIN</td><td align="center">ATTTCACACACGCAGTCGGTATCCG</td><td align="center">CCAGCAGATGGAGGAGGTC</td><td align="center">NM 013076</td></tr><tr><td align="left">PPAR<svg height="9.9375" id="M37" style="vertical-align:-2.34499pt" version="1.1" viewbox="0 0 8.0625 9.9375" width="8.0625" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.9375)">
<g transform="translate(72,-64.05)">
<text transform="matrix(1,0,0,-1,-71.95,66.44)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝛾</tspan>
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</g>
</g>
</svg></td><td align="center">TCCTCCTGTTGACCCAGAGCAT</td><td align="center">AGCTGATTCCGAAGTTGGTGG</td><td align="center">???</td></tr><tr><td align="left">LPL</td><td align="center">GAGATTTCTCTGTATGGCACA</td><td align="center">CTGCAGATGAGAAACTTTCTC</td><td align="center">NM 012598</td></tr><tr><td align="left">ADIPONECTIN</td><td align="center">AATCCTGCCCAGTCATGAAG</td><td align="center">CATCTCCTGGGTCACCCTTA</td><td align="center">NM 144744</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Primers sequences used for the determination of gene expression in adipose tissue by qRT-PCR.

Journal of Nutrition and Metabolism

tab2

Table 2

Table 2: Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat 

Table 2 | Opposing Effects of Omega-3 and Omega-6 Long Chain Polyunsaturated Fatty Acids on the Expression of Lipogenic Genes in Omental and Retroperitoneal Adipose Depots in the Rat 