Peroxidation of n-3 Polyunsaturated Fatty Acids Inhibits the Induction of iNOS Gene Expression in Proinflammatory Cytokine-Stimulated Hepatocytes
Figure 7
Effects of peroxidized EPA/DHA on the time course of iNOS protein expression. Cells were treated with IL-1β (1 nM) in the presence or absence of peroxidized EPA for 3 days and DHA for 1.5 days (each, 500 μM) for the indicated times. Cell lysates (25 μg of protein) were subjected to SDS-PAGE in a 7.5% gel, and immunoblotted with an anti-iNOS or anti-β-tubulin antibody. The bands corresponding to iNOS were quantitated by densitometry, and the levels of iNOS were normalized by those of β-tubulin (lower, mean ± SD, experiments; versus IL-1β alone).
