Transient Silencing of a Type IV P-Type ATPase, Atp10c, Results in Decreased Glucose Uptake in C2C12 Myotubes

Figure 2

C2C12 cells were differentiated from myoblasts to myotubes as described in Section 2. Myotubes were transfected at each concentration of siRNA (SI00906220) (0 and 50 nM) at the designated time point (24 h). Cells were then stimulated with insulin (100 nM, 30 min), and a 2-DOG uptake was performed (*𝑃<0.05). Data is reported as the glucose uptake stimulation which is expressed as the ratio of dpm/𝜇g of total protein in presence of insulin to that in the absence of insulin.