Research Article

The Effects of Prenatal Protein Restriction on β-Adrenergic Signalling of the Adult Rat Heart during Ischaemia Reperfusion

Figure 2

𝛽 -adrenergic receptor (AR) isoforms, Gs, and Gi protein expression in cardiac cytosolic fractions from CON and MLP Langendorff perfused rat hearts at baseline and following ischemia and ischemia reperfusion. Cardiac cytosolic expression of 𝛽 1 -AR (a), 𝛽 2 -AR (b), Gs (c), and Gi (d) was determined by western blot analysis. Data were expressed as mean ± SEM values for isolated hearts exposed to either baseline (B) perfusion, 30 min ischemia (I) or 30 min ischemia followed by 60 min reperfusion (IR) from Con Female, Con Male, or protein-restricted (MLP) MLP Female or MLP Male rats. Data were analysed by three-way ANOVA. 𝛽 1 -AR and 𝛽 2 -AR showed effects of ischemia reperfusion treatment ( 𝑃 < 0 . 0 0 1 ).
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