The Effects of Prenatal Protein Restriction on β-Adrenergic Signalling of the Adult Rat Heart during Ischaemia Reperfusion

Table 2

Catecholamine concentration levels in the urine of rats at 5 weeks, 10 weeks and 6 months of age. Urine was collected over a 24 h period and the concentration (ng/mL) of adrenaline, noradrenaline and dopamine were measured by ELISA. Adrenaline and dopamine were log transformed and Noradrenaline underwent square root transformation to produce homogeneous data. The transformed data was then analysed by three-way ANOVA and expressed as mean and SEM with corresponding  𝑃  values for each analysis.

		Adrenaline (ng/mL)		Noradrenaline (ng/mL)		Dopamine (ng/mL)
		Mean	SEM	Mean	SEM	Mean	SEM
CON Female	5 weeks	7.72	3.23	249.19	57.05	316.14	69.9
	10 weeks	23.01	4.27	128.28	75.48	294.8	83.17
	6 months	18.96	4.27	151.38	61.63	113.37	43.2
MLP Female	5 weeks	6.88	3.82	240.61	53.37	158.64	55.66
	10 weeks	14.40	3.49	51.71	75.48	107.61	29.13
	6 months	14.57	3.02	40.62	67.51	103.9	24.4
CON Male	5 weeks	6.94	3.02	177.08	57.05	116.99	14.35
	10 weeks	11.17	4.93	374.06	61.63	284.9	56.35
	6 months	12.63	3.49	59.58	75.48	276.2	72.5
MLP Male	5 weeks	8.59	4.27	82.80	61.63	163.98	61.4
	10 weeks	8.31	3.49	147.51	67.51	133.11	35.5
	6 months	8.22	3.23	98.86	61.63	148.1	56.5
ANOVA		𝑃-values		𝑃-values		𝑃-values
Diet		0.117		0.016		0.010
Sex		0.115		0.906		0.559
Age		0.072		0.022		0.400
Diet × Sex		0.662		0.842		0.880
Diet × Age		0.171		0.623		0.353
Sex × Age		0.438		0.011		0.038
Diet × Sex × Age		0.768		0.105		0.222