Figure 4: Cerebral cortex expression of TPH2 mRNA is increased in SSRI-exposed male mice. PCR was performed on midbrain and cerebral cortex homogenates obtained from control and SSRI-exposed mice (gray bars). Expression of the two tryptophan hydroxylase isoforms (TPH2 and TPH1) was normalized by GAPDH and ΔΔCT values were calculated to determine relative mRNA abundance. 𝑁 = 5 –10 mice from 5–8 litters per group; * 𝑃 < 0 . 0 5 versus control by ANOVA with the Holm-Sidak method for multiple comparisons.