Figure 5: Urinary excretion of the major melatonin metabolite is increased by neonatal SSRI exposure independent of ovarian status. Mice were placed in metabolic cages for 24 hours and urinary excretion of melatonin sulfate was quantified by ELISA for samples from control (white bars) and SSRI-exposed mice (gray bars) that had undergone sham surgery or bilateral ovariectomy at least one month prior to collection. 𝑁 = 5 –9 mice from 5–7 litters per group; * 𝑃 = 0 . 0 3 4 versus control by ANOVA, 𝑃 = 0 . 2 3 for ovariectomy versus sham surgery.