Accelerated Growth Rate Induced by Neonatal High-Protein Milk Formula Is Not Supported by Increased Tissue Protein Synthesis in Low-Birth-Weight Piglets

Table 6

Proteasome, calpain, and cathepsin L activities (RFU/min/mg protein) measured in various tissues of piglets fed NP, HP, and HPA diets at d7 and d28.

			d7					d28			Age effect
	NP	HP	HPA	SEM	P	NP	HP	HPA	SEM	P
Proteasome activity, RFU/min/mg protein
Proximal jejunum mucosa	25908	25569	26586	718	ns	31343	25827	26137	2445	ns	ns
Distal ileum mucosa	21828	23123	21803	1592	ns	26196	23846	22263	3266	ns	ns
Liver	12499	12848	12767	662	ns	13352^a	11808^b	11117^b	647	0.07	ns
Semitendinosus muscle	634	783	691	56	ns	497	376	578	67	ns	<0.001
Calpain activity, RFU/min/mg protein
Proximal jejunum mucosa	6469	6842	6794	647	ns	9307	8640	9460	925	ns	<0.001
Distal ileum mucosa	8944	9962	7819	1588	ns	12427	14015	14552	2792	ns	0.02
Liver	11505	11649	12054	914	ns	11045^a	7451^b	6884^b	948	0.01	<0.001
Semitendinosus muscle	529	505	561	92	ns	382	308	524	69	ns	0.06
Cathepsin L activity, RFU/min/mg protein
Proximal jejunum mucosa	1286	989	1136	393	ns	2511	2943	4125	978	ns	<0.01
Distal ileum mucosa	3041	2971	5642	1091	ns	2965	3019	6085	1127	ns	ns
Liver	2333	3358	2415	609	ns	3889	2301	2530	868	ns	ns
Semitendinosus muscle	965	2237	1068	113	ns	732	572	632	109	ns	<0.001

Values are least square means and pooled SEM calculated in pigs slaughtered at d7 and d28, 𝑛=6/group. P (effect of formula) and age effects are presented. Differences were considered significant when 𝑃<0.05 but trends are presented when 0.1 < 𝑃<0.05. Means with a different superscript letter differ at 𝑃<0.05.