Figure 2: Glucose incorporation into lipids in liver slices in vitro. The utilisation of glucose for esterification and de novo fatty acid synthesis was determined in the same sample as glucose incorporation into total lipids (described in Section 2). open bars = fasted animals; closed bars = fed animals. All data are means SEM, individual incubations for each bar. ***P < 0.001 HS-fasted versus SD-fasted; ††P < 0.01, †††P < 0.001 HS-fed versus SD-fed; P < 0.05 HF-fed versus SD-fed.