Timing of Maternal Exposure to a High Fat Diet and Development of Obesity and Hyperinsulinemia in Male Rat Offspring: Same Metabolic Phenotype, Different Developmental Pathways?

Table 1

Sequences of forward and reverse primers.

	Entrez gene ID	Forward primer	Reverse primer
K^+ channel (Kir6.2)	83535	5′-GAA GGA GGC AAA TGA TTG GA-3′	5′-AGT GTC CCC CAG ACA AAG TG-3′
PDE3B	29516	5′-GAC CGT CGT TGC CTT GTA TT-3′	5′-CGA TCG CCT TTC TCT ACT GG-3′
SOCS3	89829	5′-TTC TTT ACC ACC GAC GGA AC-3′	5′-CGT TGA CAG TCT TCC GAC AA-3′
Insulin1	24505	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00373303 (Qiagen)
Insulin2	24506	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00177380 (Qiagen)
Insulin receptor	24954	5′-ATC CGT CGC TCC TAT GCT CT-3′	5′-TCG TGA GGT TGT GCT TGT TC-3′
Leptin	25608	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00190960 (Qiagen)
ObRa	24536	5′-TGA TAT CGC CAA ACA GCA AA-3′	5′-AGT GTC CGC TCT CTT TTG GA-3′
ObRb	24536	5′-AAA GCC TGA AAC ATT TGA GCA TC-3′	5′-CCA GAA GAA GAG GAC CAA ATA TCA C-3′
Pdx1	29535	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00405328 (Qiagen)
STAT3	25125	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00183512 (Qiagen)
STAT5B	25126	Commercially prepared primer, QuantiTect Primer Assay, Cat. no. QT00192024 (Qiagen)
CD68	287435	Commercially prepared primer, TaqMan expression assay, Cat. no. Rn01495634 (Applied Biosystems)
TNF-α	24835	Commercially prepared primer, TaqMan expression assay, Cat. no. Rn01525859 (Applied Biosystems)
Cyclophilin	25518	5′-TTG GGT CGC GTC TGC TTC GA-3′	5′-GCC AGG ACC TGT ATG CTT CA-3′
HPRT	24465	5′-AGT CCC AGC GTC GTG ATT AG-3′	5′-CCC CCT TCA GCA CAC AGA-3′