Preliminary Studies Demonstrating Acetoclastic Methanogenesis in a Rat Colonic Ring Model

Table 2

H₂ and CH₄ production by rat colonic rings.

Gas production (ppm)^a
	H2		CH4
	1 hour	24 hours	1 hour	24 hours
Without added lactose	0	2,280 ± 1,695	0.7 ± 0.3	1.5 ± 0.4
With added lactose	0	34,280 ± 8,927	0.8 ± 0.1	6.3 ± 0.6

aOne gram of washed rat colonic rings was incubated as described in Methods. Lactose (1.25 g% final concentration) was added where indicated. H₂ and CH₄ were determined as described previously [1]. The concentration of the gases is expressed as part per million. The results are the average of three experiments, mean ± SD, with six rats in each experiment.