Journal of Nutrition and Metabolism / 2013 / Article / Fig 4

Research Article

Plant Polyphenols and Oxidative Metabolites of the Herbal Alkenylbenzene Methyleugenol Suppress Histone Deacetylase Activity in Human Colon Carcinoma Cells

Figure 4

Western blot analysis and representative western blots of HDAC1 expression in HT29 cells after 24 h treatment with polyphenols in the presence of catalase (100 U/mL) ((a) and (b)) or methyleugenol and respective metabolites ((c) and (d)). DMSO: solvent control (0.5% v/v), α-tubulin: loading control. Arbitrary light units were plotted as test over control [ ], with the respective solvent control (0.5% v/v DMSO) set to 100%. The presented data are mean ± SD of four independent experiments with similar outcome. The significances indicated were calculated in relation to the lowest applied concentration (Student’s -test: ; ).

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