A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes
Table 3
Bone densitometry of the whole body along with bone microarchitectural and biomechanical parameters of the spine and tibia.
C57BL/6J
C57BL/6N
C3H/HeJ
Con
HF
values
Con
HF
values
Con
HF
values
Whole body bone densitometry
BMC (mg)
731.2 ± 30.3
622.8 ± 23.6
0.0101
614.5 ± 24.1
492.9 ± 11.8
<0.0001
878.5 ± 25.1
810.1 ± 14.3
<0.0001
BMA (cm2)
12.31 ± 0.40
10.87 ± 0.42
0.0149
11.03 ± 0.33
8.91 ± 0.12
<0.0001
12.90 ± 0.15
11.76 ± 0.22
<0.0001
BMD (mg/cm2)
59.0 ± 0.8
57.2 ± 0.6
0.0866
55.9 ± 0.8
55.4 ± 0.6
0.6817
69.5 ± 0.7
68.6 ± 0.7
0.3777
BMD/BW [(mg/cm2)/g]
1.84 ± 0.05
1.39 ± 0.05
<0.0001
1.67 ± 0.04
1.17 ± 0.02
<0.0001
2.29 ± 0.05
1.76 ± 0.05
<0.0001
Lumbar vertebra trabecular
Conn density (1/mm3)
232.45 ± 13.37
249.77 ± 10.96
0.3398
221.21 ± 14.25
214.62 ± 16.73
0.7766
145.78 ± 18.26
116.81 ± 10.83
0.2023
SMI
0.30 ± 0.09
0.67 ± 0.02
0.0028
0.59 ± 0.10
1.00 ± 0.09
0.0136
1.10 ± 0.11
1.07 ± 0.09
0.8508
App density (mg HA/ccm)
395.86 ± 20.97
364.38 ± 7.04
0.1852
358.84 ± 7.84
318.54 ± 8.85
0.0087
279.49 ± 10.48
287.93 ± 11.99
0.6070
Mat density (mg HA/ccm)
1093.1 ± 6.7
1084.0 ± 8.9
0.4366
1078.5 ± 8.3
1076.9 ± 8.2
0.9970
1123.5 ± 10.2
1135.5 ± 11.1
0.4464
Tibial midshaft
Cortical porosity (%)
4.30 ± 0.26
4.35 ± 0.13
0.2026
4.50 ± 0.14
4.71 ± 0.10
0.8831
3.07 ± 0.08
3.69 ± 0.45
0.0505
Cortical thickness (mm)
0.17 ± 0.04
0.19 ± 0.04
0.0796
0.14 ± 0.03
0.16 ± 0.03
0.8602
0.18 ± 0.05
0.20 ± 0.04
0.1381
Cortical area (mm2)
0.011 ± 0.001
0.010 ± 0.001
0.0535
0.010 ± 0.001
0.011 ± 0.001
0.8756
0.014 ± 0.001
0.013 ± 0.001
0.3270
Medullary area (mm2)
0.035 ± 0.001
0.032 ± 0.001
0.8879
0.034 ± 0.004
0.037 ± 0.002
0.7738
0.030 ± 0.001
0.034 ± 0.001
0.0218
Indentation distance (m)
34.7 ± 1.5
32.9 ± 0.9
0.3231
29.9 ± 0.7
34.9 ± 4.5
0.2603
35.3 ± 1.1
38.4 ± 3.1
0.3587
Touchdown distance (m)
162.1 ± 12.9
150.2 ± 8.2
0.4465
148.2 ± 6.5
179.6 ± 14.7
0.0615
158.8 ± 10.8
157.6 ± 6.7
0.4269
Whole body bone densitometry and trabecular and cortical bone microarchitecture, along with reference point indentation (RPI) after 24 wk on a control (AIN-93M) or a HF (45% kcal from fat). Values are means ± SE, –15 mice per group for bone densitometry and mice per group for micro-CT data. values show the differences between dietary treatments within a given strain. Values are means ± SE.
