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Journal of Nutrition and Metabolism
Volume 2016, Article ID 6462120, 13 pages
Research Article

Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats

1Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil
2Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil
3Federal University of São Paulo (UNIFESP), Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil

Received 17 December 2015; Revised 1 April 2016; Accepted 4 April 2016

Academic Editor: Duo Li

Copyright © 2016 Maria Elizabeth Pereira Nobre et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Omega-3 (3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects of 3, in a model of global ischemia. Methods. Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups were SO, untreated ischemic and ischemic treated rats with 3 (5 and 10 mg/kg, 7 days). The SO and untreated ischemic animals were orally treated with 1% cremophor and, 1 h after the last administration, they were behaviorally tested and euthanized for neurochemical (DA, DOPAC, and NE determinations), histological (Fluoro jade staining), and immunohistochemical (TNF-alpha, COX-2 and iNOS) evaluations. The data were analyzed by ANOVA and Newman-Keuls as the post hoc test. Results. Ischemia increased the locomotor activity and rearing behavior that were partly reversed by 3. Ischemia decreased striatal DA and DOPAC contents and increased NE contents, effects reversed by 3. This drug protected hippocampal neuron degeneration, as observed by Fluoro-Jade staining, and the increased immunostainings for TNF-alpha, COX-2, and iNOS were partly or totally blocked by 3. Conclusion. This study showed a neuroprotective effect of 3, in great part due to its anti-inflammatory properties, stimulating translational studies focusing on its use in clinic for stroke managing.