Journal of Nutrition and Metabolism

Research Article

Effects of 8-Prenylnaringenin and Whole-Body Vibration Therapy on a Rat Model of Osteopenia

Table 1

Body weight, μCT, protein expression, and serum analysis data after treatment with WBVV, 8-PN, or combined therapy.


	non-OVX		OVX		OVX + VIB		OVX-8-PN		OVX-8-PN + VIB
Sample size	11		11		11		11		12
	Mean	STD	Mean	STD	Mean	STD	Mean	STD	Mean	STD

Bodyweight
Pre-OVX [g]	258.0	8.9	264.3	13.8	258.3	11.8	264.1	10.8	257.5	14.8
After 15 weeks [g]	346.6	19.1	400.7	47.4	392.5	24.0	388.3	21.3	367.0	22.1
µCT vertebrae
Trabecular number (Tb.N) []	111.5	26.98	117.5	25.56	105.4	27.27	117.6	25.45	113.8	27.91
Number of trabecular nodes N.Nd []	133.8	36.76	140.8	34.79	122.8	36.33	143.6	36.05	137.1	37.56
Mean trabecular junctions at one node (Tb.N/Nd) []	2.33	0.14	2.33	0.16	2.25	0.14	2.37	0.14	2.36	0.17
Trabecular thickness (Tb.Th) [mm]	0.05669	0.02143	0.04956	0.02006	0.04975	0.0138	0.04884	0.01932	0.04827	0.02725
BV/TV whole body of vertebrae [%]	43.82	3.22	39.84	1.94	42.66	4.46	41.68	5.82	43.32	2.92
BV/TV trabecular bone [%]	52.55	6.75	48.53	7.04	47.30	8.53	50.13	9.24	58.03	15.85
Cortical thickness (CTTh) [mm]	0.2367	0.0212	0.1977	0.0393	0.2116	0.0412	0.2042	0.0356	0.2021	0.0408
Microradiographic analysis femora
Trabecular nodes (N.Nd) []	109.1	21.7	87.5	19.2	78.4	23.1	87.0	8.7	91.6	9.9
Trabecular connectivity [N.Nd/mm²]	21.85	3.93	16.97	3.29	14.09	4.13	16.32	1.46	17.21	2.87
Trabecular bone area [mm²]	4.99	0.45	5.16	0.70	5.62	0.69	5.33	0.28	5.39	0.64
Trabecular thickness (Tb.Wi) [mm]	0.00698	0.00171	0.00597	0.00079	0.00564	0.00056	0.00553	0.00071	0.00572	0.00071
Trabecular density [%]	75.50	11.77	58.50	10.29	50.80	10.75	53.80	7.15	56.48	9.99
Cortical density [%]	97.44	1.41	96.62	1.49	97.03	0.96	96.52	0.88	96.14	1.22
Ashing
Mineral content (%)	43.00	7.59	37.82	1.66	35.97	1.54	37.46	1.68	36.00	2.10
Ca²⁺/	1.541	0.065	1.500	0.220	1.562	0.156	1.609	0.065	1.569	0.031
Gene expression
ALP	1.056	0.325	0.825	0.190	1.376	0.435	1.300	0.584	1.665	0.238
Osteocalcin	1.114	0.574	1.631	0.549	2.066	1.085	1.142	0.471	1.306	0.537
RANKL	1.199	0.556	1.956	0.310	2.938	0.874	2.054	0.633	1.661	0.816
OPG	1.218	0.968	1.296	0.732	1.595	1.066	1.472	1.756	2.187	1.801
TRAP	1.069	0.494	1.152	0.248	1.136	0.569	0.867	0.471	0.841	0.384
Serum analysis
ALP [U/I] serum	91.4	15.37	113.4	18.34	149.6	33.8	137.4	28.07	135.9	8.39

versus OVX.
versus adjunctive VIB.
versus non-OVX.