Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice

Figure 7

Effect of high fat diet and long-term treatment with EET-A on protein and mRNA expression of adipogenic markers and inflammatory cytokines. (a) Representative Western blots of FAS, aP2, and MEST proteins. Densitometric analyses of (b) FAS and (c) MEST proteins. (d) Protein and (e) mRNA levels of aP2. (f) C/EBPα, (g) TNF-α, and (h) IL-6 mRNA levels. Data are normalized to β-actin for protein or GAPDH for mRNA. Results are mean ± SE, , versus lean, and versus lean-HF.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)