Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence

Figure 1

Cytotoxicity assay (a–c) and IFN-γ secretion (d–f) of NK-92 or primary NK (pNK) effector cells [E] against DLD-1 colon tumor cells target cells [T]. (a) Cytotoxicity assay of unstimulated NK-92 cells and unstimulated pNK cells. (b and c) Cytotoxicity assay of NK-92 cells or pNK cells after leptin incubation with 10 ng/mL (L10) or 100 ng/mL (L100) leptin for 72 h. Values are expressed as means ± SEM of at least three individual experiments with each -4 well. (d) Interferon–γ (IFN-γ) secretion of unstimulated NK-92 cells and unstimulated pNK cells. (e and f) IFN-γ secretion of NK-92 cells or pNK cells after leptin incubation. Values are expressed as means ± SEM at least in three individual experiments. and , NK-92 cells compared to pNK cells; , , control group versus L10; , , and , control group versus L100.

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