Age-Related Reference Intervals for Blood Amino Acids in Thai Pediatric Population Measured by Liquid Chromatography Tandem Mass Spectrometry

Table 4

Accuracy, intra-assay, and interassay CV (%) of each amino acid at two different concentrations.

Analyte (µmol/L)	Low target	Accuracy (%)	Intra-assay CV (%)	Interassay CV (%)	High target	Accuracy (%)	Intra-assay CV (%)	Interassay CV (%)
Glycine	355	98–100	5.7	4.8	1018.5	99.5–101.6	5.7	8.3
Proline	271.5	100–101	2.8	4.5	695	62.5–100	2.1	2.5
Ornithine	219	64.9–82.4	2.5	13.1	519	65.8–85.6	1.2	11.7
Citrulline	86	88.2–94.5	2.1	9.4	313	79.5–93	2.4	7.2
Arginine	113	47.2–56.2	1.3	2.3	240	61.3–63.7	1.6	2.9
Alanine	320	100–110.2	2.5	9	563	108–138.3	7.5	11.2
Valine	159	124.5–128.4	7.1	8.2	361	85.5–106.4	9	12.2
Leucine/isoleucine	224	110.3–137	5.5	7.6	537	97.7–115.4	4	4.5
Methionine	46	99.3–100	5.2	8.6	172	100	2.3	4.5
Phenylalanine	97	106.4–115.4	2.8	5.8	420	91.3–101.8	0.7	2
Tyrosine	175	99.8–105	3.1	3.8	536	83.4–95.2	1.6	2.9
Aspartic acid	161	102.1–106.6	2	10.5	282	107.5–112.3	6.8	7.1
Glutamic acid	489	91.8–93.4	3.6	7.4	743	77.1–90.3	2.7	5.3