A Method for the Isolation of Exosomes from Human and Bovine Milk

Figure 3

Immunoblotting for the presence of exosomal marker FLOT-1 (49 kDa). (a, b) The presence of FLOT-1 in exosomes in the pooled samples (fraction 7–10) for both human and milk exosome samples. The blots also indicate presence of FLOT-1 individual exosome fractions 7, 8, 9, and 10 for both species (highlighted in yellow box). The SEC void volumes (i.e., fractions 1–6) were pooled into two groups, F1–3 and F4–6, for ease of running SDS-PAGE on a single gel. (a) Bovine. (b) Human.

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(b)