Low Citrate Synthase Activity Is Associated with Glucose Intolerance and Lipotoxicity

Table 2

Fat content in tissues of C57BL/6J (B6) and B6.A-(rs3676616-D10Utsw1)/KjnB6 (B6.A) mice after 12 weeks of high fat diet (HFD) feeding.

	Males		Females
	B6	B6.A	B6	B6.A
Fat pads (g)^††	5.818 ± 1.839	5.526 ± 1.231	4.397 ± 1.436	3.932 ± 1.290
Skin and subcutaneous fat (g)	2.614 ± 1.178	3.342 ± 1.448	2.073 ± 0.670	2.933 ± 0.726
Carcass and tail fat (g)^††	2.559 ± 0.668	2.470 ± 0.563	1.893 ± 0.651	1.832 ± 0.493
Organ fat (g)	0.311 ± 0.084	0.397 ± 0.341	0.267 ± 0.157	0.222 ± 0.062
Liver fat (g)	0.182 ± 0.174	0.115 ± 0.081	0.081 ± 0.030	0.082 ± 0.030
TA muscle fat (au)^††	15.64 ± 7.25	15.90 ± 5.59	11.39 ± 6.10	11.13 ± 3.64

Fat content of tissues was assessed using Soxhlet method for fat extractions. Intramuscular fat of the tibialis anterior (TA) muscle was evaluated using staining of the muscle cross sections with Oil Red dye with the subsequent quantification of the staining intensity. The values are means ± SD ( each). B6 vs B6.A strains; ^††, effect of sex; , effect of strain.