Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes

Figure 1

Expression of mRNA iodide transporters and inhibitory effects of lugol on mature 3T3-L1 adipocytes’ growth. (a) Expression of NIS and PEN mRNAs on undifferentiated and differentiated 3T3-L1 (mature adipocytes). Representative electrophoresis (one of the three experiments with similar results is shown). DNA ladder (1), NIS amplicon (2), NIS plus SmaI (3), PEN (4), PEN plus SacII (5), and GAPDH as internal control (6-7). (b) Mature 3T3-L1 adipocytes were treated with lugol (0, 1, 10, and 100 µM of lugol, n = 9). Cell proliferation was monitored using the MTT assay (see method) at 0.5, 6, 24 h following lugol treatment. OD λ = 570 value in mature 3T3-L1 adipocytes treated without lugol was defined as 100%. ; ; compared with the control group (vehicle). (c) Dose-dependent effects of lugol (0–1000 μg/mL) for 24 h to determine the acute cytotoxicity dose (IC50). (d) After 0.6, 6, and 24 h, cellular caspase-3 activity was measured. Fold increase in activity was calculated based on activity measured in control (vehicle) cells. Each assay represents an independent experiment performed in triplicate. Data are presented as mean ± SD. n = 5. , , and vs vehicle cells.

(a)

(b)

(c)

(d)