Undaria pinnatifida (Wakame) Intake Ameliorates High-Fat Diet-Induced Glucose Intolerance via Promoting GLUT4 Expression and Membrane Translocation in Muscle

Figure 1

Effect of wakame components on GLUT4 membrane translocation in C2C12 cells. A representative image shows the translocated GLUT4 (red) and endogenous GLUT4 (blue). Myc-GLUT4-ECFP-expressing stable C2C12 (C2C12-G4) cells were treated with (a) PBS, (b) DMSO, (c) 1 mM metformin, (d) 1 mg/mL fucoidan, (e) 50 μg/mL ethanol extract, (f) 50 μg/mL hexane extract, (g) 1 μg/mL fucoxanthinol, or (h) 100 μg/mL wakame peptide for 20 min. The C2C12-G4 cells were then fixed with 4% PFA and stained with anti-Myc antibody followed by Alexa594-labeled secondary antibody. Scale bar, 50 μm.

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